The kit uses real-time multiple fluorescent PCR technology that combined with an automated fully enclosed qPCR instrument. It is used for the in vitro qualitative detection of high-risk HPV16, HPV18 and other 16 high-risk (HPV26, HPV31, HPV33, HPV35, HPV39, HPV45, HPV51, HPV52, HPV53, HPV56, HPV58, HPV68, HPV59, HPV66, HPV73 and HPV82) human papillomavirus (HPV) nucleic acids in cervical exfoliated cells or reproductive tract secretions. The test results can be used for HPV auxiliary diagnosis.